Speaker
Descrizione
Red Blood Cell-derived Extracellular Vesicles (RBC-EVs) are biogenic nanoparticles, secreted by cells, with hemoglobin (Hb) representing almost 90% of the total protein content. [1,2] Due to their extremely high selectivity and intrinsic biocompatibility, EVs are currently regarded as the most promising platform for drug delivery in several medical research fields such as personalized cancer treatment. [3] In this work, we present the current progress of our spectro-microscopy investigation of RBC-EVs loaded with the chemotherapy drug cisplatin (CisPt). [4,5,6] This drug can strongly interact with hemoglobin, promoting the formation of Hb−Pt complexes and the release of the heme group. [7] To highlight the localization of CisPt inside the RBC-EVs, and to probe the occurrence of chemical interactions, we have performed XANES investigation with spatial resolution of about 50 nm at the I14 beamline of the Diamond Light Source. Preliminary fine‑tuning of surface decoration was performed at ISMN through iterative substrate functionalization, EV deposition, and scanning probe microscopy, ultimately converging on samples optimally suited for measurements at I14. Our results, which are challenging to obtain with conventional techniques used for the EVs, could significantly enhance the understanding of the cisplatin loading process and its mechanism of action, thus paving the way for future advancements in the application of RBC-EVs.
References
[1] K. Thangaraju et al., Int. J. Mol. Sci. 22(1), 153 (2021).
[2] S. Biagiotti et al., Pharmaceutics 15(2), 365 (2023).
[3] N.D. Eliack et al., Metallomics 6, 2126–2133 (2014).
[4] A. Musicò et al., Nanoscale Adv. 5, 4703–4717 (2023).
[5] A. Ridolfi et al., J. Extracell. Vesicles 12, 2022.07.19.500441 (2023).
[6] E. Schulz et al., European Journal of Pharmaceutics and Biopharmaceutics 146 (2020), 55.
[7] R. Mandal et al., Chem. Res. Toxicol. 17, 1391–1397 (2004).
| Consenso all'uso dei dati | Acconsento che l'abstract possa essere incluso nel programma del convegno e nel libro degli abstract. Acconsento al trattamento dei dati personali per finalità organizzative del convegno, in conformità al GDPR. |
|---|---|
| Giovane Ricercatore (under 40) | No |
